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Human R-Spondin 1 (RSPO1) Protein (Active)
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Catalogue No: abx691604
Price:
US$1,276.00
(Size: 100 µg)
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Human R-Spondin 1 Protein is a recombinant protein from Human produced in HEK293 Cells. A DNA sequence encoding the human RSPO1 (NP_001033722.1) (Met 1-Ala 263) was expressed, fused with a polyhistidine tag at the C-terminus.
Research Articles on R-Spondin 1 (RSPO1)
| Target | R-Spondin 1 (RSPO1) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | SDS-PAGE |
| Host | HEK293 cells |
| Form | Lyophilized |
| Purity | > 95% (SDS-PAGE) |
| Reconstitution | Reconstitute in sterile distilled water to a concentration of 0.1 mg/ml. Do not vortex. |
| Storage | Store lyophilized at -20 °C or -80 °C. Upon reconstitution, store between 4-8 °C for up to 7 days, or aliquot and store at or below -20 °C for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Validity | The validity for this protein is 12 months. |
| UniProt Primary AC | Q2MKA7 (UniProt, ExPASy) |
| UniProt Secondary AC | A2A420, Q0H8S6, Q14C72, Q5T0F2, Q8N7L5 |
| UniProt Entry Name | RSPO1_HUMAN |
| NCBI Accession | NP_001033722.1 |
| Molecular Weight | Calculated MW: 28.2 kDa Observed MW (SDS-PAGE): 42 kDa The difference between the calculated MW and observed MW is due to glycosylation. |
| Sequence Fragment | Met1-Ala263 |
| Sequence | MRLGLCVVAL VLSWTHLTIS SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA |
| Tag | C-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4. |
| Activity | Active |
| Biological Activity | The biological activity was determined by measuring the binding ability in a functional ELISA. Immobilized human RSPO1 (20 μg/ml, 100 μl/well) can bind human LIMPII with a linear range of 32 ng/ml - 800 ng/ml. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind mouse CD36 with a linear range of 6.4 ng/ml - 800 ng/ml. The biological activity was determined by measuring the ability to induce activation of beta catenin response in a TOPFlash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 μg/ml in the presence of recombinant mouse Wnt3a (5 ng/ml). |
| Endotoxin Level | < 1.0 EU per µg as determined by the LAL method. |
| Availability | Shipped within 5-15 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
| Directions for use | Centrifuge before opening to ensure complete recovery of vial contents. |