Rabbit IgG SABC Kit (Peroxidase)

Rabbit IgG SABC Kit (Peroxidase) is designed for use with Rabbit primary antibodies in IHC and ICC. This kit contains a biotin-conjugated Goat anti-Rabbit secondary antibody and peroxidase-conjugated streptavidin. A streptavidin-biotin complex (SABC) is formed since streptavidin has high affinity for biotin. Since streptavidin exhibits very low non-specific binding to tissues and cells, due to its nearly neutral isoelectric point, this kit can be used for low background immunohistochemical analysis. This kit also has high sensitivity due to the large number of peroxidase and streptavidin molecules used.

Kit Components:

• 5% BSA: 12 ml
• Goat anti-Rabbit Antibody (Biotin): 12 ml
• Streptavidin (Peroxidase): 12 ml

Material Required But Not Provided:

1. APES or Poly-L-Lysine
2. 0.02 M PBS (pH 7.2-7.6): dissolve 8.5 g NaCl, 2.8 g anhydrous Na₂HPO₄ and 0.4 g anhydrous NaH₂HPO₄ in 1 L of distilled water. (If hydrous phosphates are used, adjust the weight accordingly)
3. 0.01 M Citrate Buffer: dissolve 3 g sodium citrate dihydrate (C₆H₅Na₃O₇ · 2H₂O) and 0.4 g citric acid monohydrate (C₆H₈O₇ · H₂O).
4. DAB Horseradish Peroxidase Chromogenic Kit (abx090660)
5. 0.1% Trypsinase, or other compound digest solution

Target	Rabbit IgG
Tested Applications	IHC, IF/ICC
Form	Liquid
Storage	Store at 2°C to 8°C for up to one year.
Buffer	Contains BSA.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Rabbit IgG

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