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Raf Proto-Oncogene Serine/Threonine Protein Kinase (RAF1) Antibody
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Catalogue No: abx015975
Price:
US$420.50
(Size: 100 µl)
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This gene is the cellular homolog of viral raf gene (v-raf).The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly.Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2.Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Research Articles on Raf Proto-Oncogene Serine/Threonine Protein Kinase (RAF1)
| Target | Raf Proto-Oncogene Serine/Threonine Protein Kinase (RAF1) |
| Clonality | Monoclonal |
| Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, FCM |
| Host | Mouse |
| Recommended dilutions | ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Purified recombinant fragment of human RAF1 expressed in E. coli. |
| Isotype | IgG1 |
| Form | Liquid |
| Purification | Purified from ascites by Protein G chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P04049 (UniProt, ExPASy) |
| UniProt Secondary AC | B0LPH8, B2R5N3, Q15278, Q9UC20 |
| UniProt Entry Name | RAF1_HUMAN |
| Gene Symbol | RAF1 |
| GeneID | 5894 |
| OMIM | 164760 |
| HGNC | 9829 |
| KEGG | hsa:5894 |
| Ensembl | ENSG00000132155 |
| String | 9606.ENSP00000251849 |
| Molecular Weight | 73 kDa |
| Buffer | PBS, containing 0.05% sodium azide. |
| Concentration | 1 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |