Rat Coagulation Factor II, Thrombin / Prothrombin (F2) Protein

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Catalogue No: abx065954
Price: US$275.50
(Size: 10 µg)

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Datasheet SDS
Rat Coagulation Factor II (F2) is a recombinant Rat protein produced in a Prokaryotic expression system (E. coli).

This protein is the immunogen for the following antibodies: abx101853

Target Coagulation Factor II, Thrombin / Prothrombin (F2)
Origin Rat
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 97%
Reconstitution To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P18292 (UniProt, ExPASy)
UniProt Entry Name THRB_RAT
String 10116.ENSRNOP00000022233
Molecular Weight Calculated MW: 18.1 kDa
Observed MW (SDS-PAGE): 23 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Ala44-Arg200
Sequence ANSGFLE ELRKGNLERE CVEEQCSYEE AFEALESPQD TDVFWAKYTV CDSVRKPRET FMDCLEGRCA MDLGLNYHGN VSVTHTGIEC QLWRSRYPHR PDINSTTHPG ADLKENFCRN PDSSTSGPWC YTTDPTVRRE ECSIPVCGQE GRTTVKMTPR
Tag N-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.
Activity Not tested
Concentration Prior to lyophilization: 3500 µg/ml
Availability Shipped within 5-12 working days.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Research Articles on Coagulation Factor II, Thrombin / Prothrombin (F2)


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