Rat Coagulation Factor IX (F9) Protein

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Catalogue No: abx065961
Price: US$261.00
(Size: 10 µg)

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Datasheet SDS
Rat Coagulation Factor IX (F9) is a recombinant Rat protein produced in a Prokaryotic expression system (E. coli).

Target Coagulation Factor IX (F9)
Origin Rat
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 90%
Reconstitution To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P16296 (UniProt, ExPASy)
UniProt Secondary AC F1M1M6
UniProt Entry Name FA9_RAT
Gene Symbol F9
GeneID 24946
KEGG rno:24946
String 10116.ENSRNOP00000004559
Molecular Weight Calculated MW: 23.8 kDa
Observed MW (SDS-PAGE): 27 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Asn61-Glu263
Sequence NAKPGQIPWQ VILNGEIEAF CGGAIINEKW IVTAAHCLKP GDKIEVVAGE HNIDEKEDTE QRRNVIRTIP HHQYNATINK YSHDIALLEL DKPLILNSYV TPICVANKEY TNIFLKFGSG YVSGWGKVFN KGRQASILQY LRVPLVDRAT CLRSTKFSIY NNMFCAGYRE GGKDSCEGDS GGPHVTEVEG TSFLTGIISW GEE
Tag N-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.
Activity Not tested
Concentration Prior to lyophilization: 250 µg/ml
Availability Shipped within 5-12 working days.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Research Articles on Coagulation Factor IX (F9)


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