Rat Glutamate Decarboxylase 2 (GAD2) Protein

Glutamate Decarboxylase 2 (GAD2) is a recombinant Rat protein produced in a Prokaryotic expression system (E. coli). This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Target	Glutamate Decarboxylase 2 (GAD2)
Origin	Rat
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 95%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q05683 (UniProt, ExPASy)
UniProt Entry Name	DCE2_RAT
KEGG	rno:24380
String	10116.ENSRNOP00000024901
Molecular Weight	Calculated MW: 21.9 kDa
Sequence Fragment	Met1-Ala150
Sequence	MASPGSGFWS FGSEDGSGDP ENPGTARAW C QVAQKFTGGI GNKLCALLYG DSEKPAESGG SVTSRAATRK VACTCDQKPC SCPKGDVNYA LLHATDLLPA CEGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA
Tag	N-terminal His tag
Buffer	Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
Activity	Not tested
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 1-2 months.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on Glutamate Decarboxylase 2 (GAD2)

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