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Rat Gremlin 1 (GREM1) Protein (Active)
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Catalogue No: abx651289
Price:
US$681.50
(Size: 50 µg)
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Recombinant Gremlin 1 (GREM1) is an active Rat protein produced in a Prokaryotic expression system (E. coli).
Research Articles on Gremlin 1
| Target | Gremlin 1 |
| Origin | Rat |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 95% |
| Reconstitution | Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| Molecular Weight | Calculated MW: 22.0 kDa Observed MW: 24 kDa (determined by SDS-PAGE) |
| Sequence Fragment | Lys25-Asp184 |
| Sequence | KKKGSQ GAIPPPDKAQ HNDSEQTQSP PQPGSRTRGR GQGRGTAMPG EEVLESSQEA LHVTERKYLK RDWCKTQPLK QTIHEEGCNS RTIINRFCYG QCNSFYIPRH IRKEEGSFQS CSFCKPKKFT TMMVTLNCPE LQPPTKKKRV TRVKQCRCIS IDLD |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 100 mM NaHCO3, 500 mM NaCl, pH 8.3, containing 1 mM DTT, 0.01% sarcosyl, 5% trehalose and Proclin-300. |
| Activity | Active |
| Biological Activity | GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. A proliferation assay was conducted to detect the bioactivity of recombinant rat GREM1 using A549 cells. Briefly, A549 cells were seeded into triplicate wells in 96-well plates at a density of 5,000 cells/well and allowed to attach with serum-free medium overnight, then the medium was replaced with 2% serum standard DMEM prior to the addition of various concentrations of GREM1. After incubation for 96 h, cells were observed by inverted microscope (Figures 3 and 4) and cell proliferation was measured by Cell Counting Kit-8 (CCK-8) assay (Figure 5). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C. |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |