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Target | Immunoglobulin M (IgM) |
Origin | Rat |
Expression | Recombinant |
Tested Applications | WB, SDS-PAGE |
Host | E. coli |
Conjugation | Unconjugated |
Form | Lyophilized |
Purity | > 80% |
Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
Molecular Weight | Calculated MW: 81.5 kDa Observed MW (SDS-PAGE): 82 kDa |
Sequence Fragment | Tyr2-Asn467 |
Sequence | YYWYFDFWG PGTMVTVSSE SQSSPTVFPL VSCESPLSDE NLVAMGCLAR DFLPSSISFS WNYQNNTEVM QGVRTFPTLR TGDKYTATSQ VLLSAKNVLE GSDEYLVCKI HHGNKNKDLH VPIPAVVEMN PNVSVFIPPR DAFSGPAPRK SRLICEATNF SPKQITVSWL QDGKPVKSGF TTEPVTVEAK GSRPQTYKVI STLTITESDW LNLNVFTCRV DHRGLTFWKN VSSTCAASPS TDILAFPIPP SFADIFLTKS AKLSCLVTNL ATYDTLNISW SSKSGEPLET NTKIMESHPN GTFSAVGVAS VCMEDWDNRK EFVCTVTHRD LPSPQKKFIS KPNEVAKHPP AVYLLPPARE QLILRESATV TCLVKGFSPA DIFVQWLQRG QPLSSDKYVT SAPMPEPGAP GLYFTHSILT VTEEEWNSGE TYTCVVGHEA LPHMVTERTV DKSTEGEVNA EEEGFEN |
Tag | N-terminal His tag and GST tag |
Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
Activity | Not tested |
Concentration | Prior to lyophilization: 150 µg/ml |
Availability | Shipped within 5-12 working days. |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |