Rat Osteopontin (SPP1) Protein

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Catalogue No: abx654632
Price: US$261.00
(Size: 10 µg)

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Datasheet SDS
Rat Osteopontin (SPP1) Protein is a Recombinant Rat protein expressed in E. coli.

Target Osteopontin (SPP1)
Origin Rat
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 90%
Reconstitution Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P08721 (UniProt, ExPASy)
UniProt Entry Name OSTP_RAT
Gene Symbol SPP1
GeneID 25353
KEGG rno:25353
String 10116.ENSRNOP00000062358
Molecular Weight Calculated MW: 36.7 kDa
Observed MW: 16/60 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Leu17-Asn317
Sequence LPVK VAEFGSSEEK AHYSKHSDAV ATWLKPDPSQ KQNLLAPQNS VSSEETDDFK QETLPSNSNE SHDHMDDDDD DDDDGDHAES EDSVNSDESD ESHHSDESDE SFTASTQADV LTPIAPTVDV PDGRGDSLAY GLRSKSRSFP VSDEQYPDAT DEDLTSRMKS QESDEAIKVI PVAQRLSVPS DQDSNGKTSH ESSQLDEPSV ETHSLEQSKE YKQRASHEST EQSDAIDSAE KPDAIDSAER SDAIDSQASS KASLEHQSHE FHSHEDKLVL DPKSKEDDRY LKFRISHELE SSSSEVN
Tag N-terminal His tag
Buffer Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Activity Not tested
Concentration Prior to lyophilization: 100 µg/ml
Availability Shipped within 5-7 working days.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Research Articles on Osteopontin (SPP1)


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