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Target | Osteopontin (SPP1) |
Origin | Rat |
Expression | Recombinant |
Tested Applications | WB, SDS-PAGE |
Host | E. coli |
Conjugation | Unconjugated |
Form | Lyophilized |
Purity | > 90% |
Reconstitution | Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P08721 (UniProt, ExPASy) |
UniProt Entry Name | OSTP_RAT |
Gene Symbol | SPP1 |
GeneID | 25353 |
KEGG | rno:25353 |
String | 10116.ENSRNOP00000062358 |
Molecular Weight | Calculated MW: 36.7 kDa Observed MW: 16/60 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
Sequence Fragment | Leu17-Asn317 |
Sequence | LPVK VAEFGSSEEK AHYSKHSDAV ATWLKPDPSQ KQNLLAPQNS VSSEETDDFK QETLPSNSNE SHDHMDDDDD DDDDGDHAES EDSVNSDESD ESHHSDESDE SFTASTQADV LTPIAPTVDV PDGRGDSLAY GLRSKSRSFP VSDEQYPDAT DEDLTSRMKS QESDEAIKVI PVAQRLSVPS DQDSNGKTSH ESSQLDEPSV ETHSLEQSKE YKQRASHEST EQSDAIDSAE KPDAIDSAER SDAIDSQASS KASLEHQSHE FHSHEDKLVL DPKSKEDDRY LKFRISHELE SSSSEVN |
Tag | N-terminal His tag |
Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose. |
Activity | Not tested |
Concentration | Prior to lyophilization: 100 µg/ml |
Availability | Shipped within 5-7 working days. |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |