Rat Prostate Fibroblasts (PF)

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Catalogue No: abx700217
US$1,493.50 US$746.75
(Size: 5 × 10^5 cells)
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Morphology of Rat Prostate Fibroblasts (Optical microscope, 100X).
Rat Prostate Fibroblasts (PF) Rat Prostate Fibroblasts (PF)

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Datasheet SDS
Rat Prostate Fibroblasts (PF) are Adherent Rat Fibroblasts from Rat Prostate.

Target Prostate Fibroblasts (PF)
Origin Rat
Host Rat
Purity Negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
Storage Shipped at -70 °C. Upon receipt, store in liquid nitrogen. Avoid repeated freeze/thaw cycles.
Validity 12 months.
Buffer Contains 90% FBS and 10% DMSO.
Biological Activity Cell activity: > 85% (viability by Trypan Blue exclusion)
Availability Shipped within 7-15 working days.
Note This product is for research use only. This product is shipped with dry ice.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Directions for use

Recommended Cell Culture Conditions:

DMEM + 10% FBS + Rat Fibroblast growth supplement + 1% Penicillin-Streptomycin Solution @ 37 °C, 95% air, 5% CO2.

Cell Recovery: Thaw cells in a 37 °C water bath with shaking until the mixture has dissolved. Transfer to a centrifuge tube and add culture medium (see Recommended Cell Culture Conditions above) at a volume 3-5 times the volume of the cells. Centrifuge at 1000 RPM for 5 minutes and discard the supernatant. Transfer to a T25 flask for culture.

Suggested Cell Passage Procedure: Cells should be 85-95% confluent before cell passage is carried out.

  1. Discard the medium and wash with PBS 1-2 times.
  2. Add 1 ml of Trypsin at 37 °C, then observe the cells under a microscope.
  3. When the cells appear retracted and rounded, gently tap the culture flask to detatch the cells. Stop the trypsinization by adding 2 ml of culture medium containing 10% serum.
  4. Add fresh medium to resuspend the cells. The recommended ratio of primary cells is 1/2. Pipette to obtain a single cell suspension.
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