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Rb-like-1 (pS975) Antibody
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Catalogue No: abx031905
Price:
US$326.25
(Size: 80 µl)
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RB-like 1 is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and RB-like 1 can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that RB-like 1 may also be a tumor suppressor.
Research Articles on Rb-like-1 (pS975)
| Target | Rb-like-1 (pS975) |
| Clonality | Polyclonal |
| Target Modification | Ser975 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Host | Rabbit |
| Recommended dilutions | WB: 1/250, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S975 of human Rb-like-1. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P28749 (UniProt, ExPASy) |
| UniProt Secondary AC | A8K2W5, Q4VXA0, Q8N5K6, Q9H1L5, Q9H1M1 |
| UniProt Entry Name | RBL1_HUMAN |
| KEGG | hsa:5933 |
| String | 9606.ENSP00000362768 |
| Molecular Weight | Calculated MW: 121 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Mouse and Rat RBL1. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |