Transcription Factor P65 (RELA) Antibody

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1) /p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

Target	Transcription Factor P65 (RELA)
Clonality	Polyclonal
Reactivity	Human, Mouse
Tested Applications	ELISA, WB, IHC, IF/ICC, FCM
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100, IF/ICC: 1/10 - 1/50, FCM: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 10-37 amino acids from the N-terminal region of human RELA.
Isotype	IgG
Form	Liquid
Purification	Purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q04206 (UniProt, ExPASy) Q04207 (UniProt, ExPASy) P98152 (UniProt, ExPASy) Q04865 (UniProt, ExPASy)
Gene Symbol	RELA
GeneID	5970
NCBI Accession	NP_001138610.1 NP_001230913.1 NP_068810.3
Molecular Weight	Calculated MW: 60.2 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Chicken and Xenopus RELA.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Transcription Factor P65 (RELA)

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