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Transcription Factor p65 / NFKB3 (RELA) Antibody
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Catalogue No: abx033005
Price:
US$326.25
(Size: 80 µl)
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NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1) /p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
Research Articles on Transcription Factor p65 / NFKB3 (RELA)
| Target | Transcription Factor p65 / NFKB3 (RELA) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC, IF/ICC, FCM |
| Host | Rabbit |
| Recommended dilutions | WB: 1/1000, IHC-P: 1/50 - 1/100, IF/ICC: 1/10 - 1/50, FCM: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic peptide between 10-37 amino acids from the N-terminal region of human RELA. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q04206 (UniProt, ExPASy) |
| UniProt Secondary AC | Q6GTV1, Q6SLK1 |
| UniProt Entry Name | TF65_HUMAN |
| Gene Symbol | RELA |
| GeneID | 5970 |
| OMIM | 164014 |
| NCBI Accession | NP_001138610.1 NP_001230913.1 NP_068810.3 |
| HGNC | 9955 |
| KEGG | hsa:5970 |
| Ensembl | ENSG00000173039 |
| String | 9606.ENSP00000384273 |
| Molecular Weight | Calculated MW: 60.2 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Chicken and Xenopus RELA. |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |