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Target | Rubella Grade IV Antigen (HPV77) |
Origin | Virus |
Tested Applications | ELISA |
Host | Monkey |
Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | Unconjugated |
Form | Liquid |
Purification | Purified from Vero Cells by gradient purification. Inactivated by UV irradiation and verified by infectivity testing (Plaque Assay: 0 pfu/ml). |
Storage | Aliquot and store at or below -65 °C. Avoid repeated freeze/thaw cycles. |
Buffer | 10 mM Tris, pH 8.0-8.4, containing 150 mM NaCl, 1.0 mM EDTA. Does not contain preservatives. |
Biological Activity | ELISA Midpoint Titer: Specification: -15% to +50% of Reference Antigen Reference Antigen: 207.3 Test Antigen: 300.2 % of Reference Antigen: 44.81% Background ELISA Ratio: Specification: ≤ 2 Result: 1 Sensitivity/Specificity Negative Control Result: 1 Sensitivity/Specificity Medium Positive Control Specification: ≥ 3 Result: 9 Sensitivity/Specificity High Positive Control Specification: ≥ 8 Result: 18 |
Concentration | BCA Assay: Specification: ≥ 0.250 mg/ml Result: 0.299 mg/ml OD 260/280 nm Assay: Specification: 0.45-0.60 mg/ml Result: 0.55 mg/ml |
Availability | Shipped within 10-20 working days. |
Note | This product is for research use only. This product is shipped with dry ice. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Directions for use | Centrifuge before opening to ensure complete recovery of vial contents. It is recommended to sonicate using a microprobe set to 10% amplitude, applying 1-3 second pulses with the sample on ice. |