S100 Calcium Binding Protein A11 (S100A1) Antibody

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Catalogue No: abx227093
Price: US$304.50
(Size: 40 µl)
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IHC-P analysis of S-100 protein expression in the cutaneous malignant melanoma (4 µm section).
S100 Calcium Binding Protein A11 (S100A1) Antibody

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Datasheet SDS
S100 Calcium Binding Protein A11 (S100A1) Antibody is a Rabbit Monoclonal antibody for the detection of S-100.

Target S100 Calcium Binding Protein A11 (S100A1)
Clonality Monoclonal
Clone I371
Reactivity Human
Tested Applications IHC
Host Rabbit
Recommended dilutions IHC-P: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Synthetic peptide derived from the C-terminal region of human S100-A1 protein.
Isotype IgG
Form Liquid
Purification Purified from rabbit antiserum by proprietary techniques.
Storage Store at 2-8°C.
UniProt Primary AC P23297 (UniProt, ExPASy)
Buffer 20 mM Tris-HCl, pH 8.0, containing 20 mg/ml BSA and 0.05% NaN3.
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Directions for use Suggested IHC-P Protocol
  1. Preparation of Wash Buffer: Use 0.05 M Tris-HCl, pH 7.6, containing 1% Tween-20.
  2. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
  3. Wash the section in 96%, 80% and 70% ethanol, 10 minutes each.
  4. Rinse in distilled water.
  5. Block endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
  6. Wash in distilled water for 5 minutes.
  7. Wash in Wash Buffer for 5 minutes.
  8. Incubate the section with primary antibody at 1/100 - 1/200 dilution for 1 hour in a closed wet chamber. It is recommended to use abx291502 Primary Antibody Diluent or a diluent containing protease-free BSA (> 1 mg/ml) to dilute this antibody.
  9. Wash twice with Wash Buffer, 5 minutes each.
  10. Add the secondary antibody and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). It is recommended to use abx291501 Rabbit and Mouse HRP/DAB Detection Kit.
  11. Wash twice with Wash Buffer, 5 minutes each.
  12. Apply the DAB chromagen for 1-3 minutes.
  13. Wash in distilled water for 10 minutes.
  14. Stain in hematoxylin for 5 minutes.
  15. Wash in distilled water for 10 minutes.
  16. Dehydrate the section in 2 changes of 96% ethanol, 5 minutes each.
  17. Wash the section in 2 changes of xylene, 2 minutes each.
  18. Mount the slide for observation.
Research Articles on S100 Calcium Binding Protein A11 (S100A1)


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