S100 Calcium Binding Protein A6 (S100A6) Protein

S100 Calcium Binding Protein A6 (S100A6) Protein (Active) is an active protein from Human.

Target	S100 Calcium Binding Protein A6 (S100A6)
Origin	Human
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 92%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P06703 (UniProt, ExPASy)
UniProt Secondary AC	D3DV39, Q5RHS4
UniProt Entry Name	S10A6_HUMAN
KEGG	hsa:6277
String	9606.ENSP00000357709
Molecular Weight	Calculated MW: 16.9 kDa Observed MW: 16 kDa
Sequence Fragment	Met1-Gly90
Sequence	MACPLDQAIG LLVAIFHKYS GREGDKHTLS KKELKELIQK ELTIGSKLQD AEIARLMEDL DRNKDQEVNF QEYVTFLGAL ALIYNEALKG
Tag	N-terminal His tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Activity	Active
Biological Activity	Human S100 Calcium Binding Protein A6 (S100A6) is a calcium-binding protein that functions as calcium sensor and modulator, contributing to cellular calcium signaling. It is reported that S100B and S100A6 differentially modulate cell survival by interacting with RAGE (Receptor for Advanced Glycation End Products, also known as AGER). Therefore a binding ELISA assay was conducted to detect the interaction of S100A6 and RAGE. Briefly, S100A6 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to RAGE-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-S100A6 monoclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of S100A6 and RAGE is shown in Figure 3.
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on S100 Calcium Binding Protein A6 (S100A6)

Human S100 Calcium Binding Protein A6 (S100A6) Protein (Active)

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