N-Terminal Kinase-Like Protein (SCYL1) Antibody

SCYL1 forms multimers following transfection into COS-7 cells. SCYL1 forms a 300-kD trimer using crosslinking reagents. Biochemical analysis revealed no phosphorylation or autophosphorylation activity.The 707-amino acid SCYL1 variant, variant 2, localized to centrosomes during mitosis. During interphase, fluorescence-tagged variant 2 localized in the cytoplasm as well as centrosomes. However, at the beginning of mitosis, the fluorescence appeared as a pair of bright nuclear foci that followed centrosome localization throughout mitosis, while maintaining diffuse cytoplasmic labeling. Endogenous variant 2 in HeLa cells showed a similar staining pattern. Centrosomal localization was independent of microtubules.

Target	N-Terminal Kinase-Like Protein (SCYL1)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 156-185 amino acids from the N-terminal region of human SCYL1.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein A column, followed by peptide affinity purification.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q96KG9 (UniProt, ExPASy)
UniProt Secondary AC	A6NJF1, Q96G50, Q96KG8, Q96KH1, Q9HAW5, Q9HBL3, Q9NR53
UniProt Entry Name	SCYL1_HUMAN
Gene Symbol	SCYL1
KEGG	hsa:57410
String	9606.ENSP00000270176
Molecular Weight	Calculated MW: 89.6 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Cow SCYL1.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on N-Terminal Kinase-Like Protein (SCYL1)

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