SDS-PAGE Loading Buffer (2X)

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Catalogue No: abx090647
Price: US$130.50
(Size: 5 ml)
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SDS-PAGE Loading Buffer (2X)

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Datasheet SDS
SDS-PAGE Sample Loading Buffer is a 2X concentrated solution based on Bromophenol blue dye for use in SDS-PAGE protein sample loading.

Tested Applications WB
Storage Store at -20 °C for up to one year.
Buffer Contains Tris-HCl buffer, SDS, DTT, Bromophenol Blue and glycerol.
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use
  1. Allow to stand in a water bath at a temperature between room temperature and 37 °C until all precipitates/sediments have dissolved. Then, allow to stand at room temperature.
  2. Add sample to the 2X SDS-PAGE Sample Loading Buffer in a ratio of 1:1 (e.g. add 1 µl of sample to 1 µl of 2X SDS-PAGE Sample Loading Buffer).
  3. Fully denature the sample at 100 °C or in a boiling water bath for 3-5 min.
  4. Cool the sample to room temperature, then load and carry out electrophoresis.
  5. Stop electrophoresis when the bromophenol blue dye reaches the bottom of the gel.

Notes:
  • If SDS sediments appear in the SDS-PAGE Sample Loading Buffer at room temperature, they can be dissolved using a warm water bath.
  • This product contains DTT, which can have a distinct odour.
  • Personal protective equipment (PPE), such as lab coats, lab glasses and disposable gloves, are recommended when using this product.
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