SDS-PAGE Loading Buffer (5X)

REQUEST MORE INFO
Catalogue No: abx090648
Price: US$145.00
(Size: 5 ml)
Reviews: 0 Publications and Reviews
(0 Publications and Reviews)

Click on the image to see the image legend

SDS-PAGE Loading Buffer (5X)

Available Options

* Size:


Add to Shopping Cart Buy It Now GET A QUOTE

Documents

Datasheet SDS
SDS-PAGE Sample Loading Buffer is a 5X concentrated solution based on Bromophenol blue dye for use in SDS-PAGE protein sample loading.

Tested Applications WB
Storage Store at -20 °C for up to one year.
Buffer Contains Tris-HCl buffer, SDS, DTT, Bromophenol Blue and glycerol.
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Directions for use
  1. Allow to stand in a water bath at a temperature between room temperature and 37 °C until all precipitates/sediments have dissolved. Then, allow to stand at room temperature.
  2. Add sample to the 5X SDS-PAGE Sample Loading Buffer in a ratio of 4:1 (e.g. add 4 µl of sample to 1 µl of 5X SDS-PAGE Sample Loading Buffer).
  3. Fully denature the sample at 100 °C or in a boiling water bath for 3-5 min.
  4. Cool the sample to room temperature, then load and carry out electrophoresis.
  5. Stop electrophoresis when the bromophenol blue dye reaches the bottom of the gel.

Notes:
  • If SDS sediments appear in the SDS-PAGE Sample Loading Buffer at room temperature, they can be dissolved using a warm water bath.
  • This product contains DTT, which can have a distinct odour.
  • Personal protective equipment (PPE), such as lab coats, lab glasses and disposable gloves, are recommended when using this product.
Research Articles on SDS-PAGE Loading Buffer (5X)


Write a review

Continue
Tags:
(Click to show)