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SEPARIN (pS1126) Antibody
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Catalogue No: abx031912
Price:
US$326.25
(Size: 80 µl)
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The metaphase-to-anaphase transition is the final discrete event in duplication and separation of the genetic material of the cell. Its timing is regulated by the activation of the anaphase-promoting complex (APC). In both budding and fission yeast, the degradation of the Pds1 or Cut2 protein, respectively, is required for the onset of sister chromatid separation. Both proteins are APC substrates. Pds1 and Cut2 proteins associate with the yeast separin proteins Esp1 and Cut1, respectively, and prevent the separins from promoting chromatid separation. Pds1 and Cut2 are also called anaphase inhibitors or securins.
Research Articles on SEPARIN (pS1126)
| Target | SEPARIN (pS1126) |
| Clonality | Polyclonal |
| Target Modification | Ser1126 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, IHC, DB |
| Host | Rabbit |
| Recommended dilutions | IHC-P: 1/50 - 1/100, DB: 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1126 of human SEPARIN. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q14674 (UniProt, ExPASy) |
| UniProt Entry Name | ESPL1_HUMAN |
| NCBI Accession | NP_036423.4 |
| KEGG | hsa:9700 |
| String | 9606.ENSP00000257934 |
| Molecular Weight | Calculated MW: 233 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |