Histone methyltransferases (HMTases) selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4. Signal transduction pathways affecting the N-terminal tails of histones lead to a number of post-translational modifications including acetylation, phosphorylation, poly (ADP-ribosylation), ubiquitination and methylation. These modifications play critical roles in regulating chromatin structure and gene expression. Set7/9 is a histone specific HMTase that methylates histone H3 lysine 4. Set7/9 transfers methyl groups to lysine 4 of histone H3 in complex with S-adenosyl-L-methionine. In yeast, H4-K20 methylation does not have any apparent role in the regulation of gene expression or heterochromatin function; rather it appears to play a role in DNA damage response. Loss of Set9 activity or mutation of H4-K20 markedly impairs yeast cell survival after genotoxic challenge and compromises the ability of cells to maintain checkpoint mediated cell cycle arrest. Genetic experiments link Set9 to Crb2, a homolog of the mammalian checkpoint protein 53BP1, and the enzyme is required for Crb2 localization to sites of DNA damage.
Target |
Histone-Lysine N-Methyltransferase SETD7 (SET7) |
Clonality |
Polyclonal |
Reactivity |
Human |
Tested Applications |
ELISA, WB |
Host |
Rabbit |
Recommended dilutions |
WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation |
Unconjugated |
Immunogen |
KLH-conjugated synthetic peptide between 289-317 amino acids from the C-terminal region of human SET7. |
Isotype |
IgG |
Form |
Liquid |
Purification |
Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC |
Q8WTS6 (UniProt,
ExPASy)
|
UniProt Secondary AC |
B5WWL3, Q0VAH3, Q4W5A9, Q9C0E6 |
UniProt Entry Name |
SETD7_HUMAN |
KEGG |
hsa:80854
|
String |
9606.ENSP00000274031
|
Molecular Weight |
Calculated MW: 40.7 kDa |
Buffer |
PBS containing 0.09% sodium azide. |
Availability |
Shipped within 5-10 working days. |
Note |
This product is for research use only. |
Research Articles on Histone-Lysine N-Methyltransferase SETD7 (SET7)