Single-Luciferase Reporter Assay Kit

REQUEST MORE INFO
Catalogue No: abx098133
Price: US$754.00
(Size: 50 rxns)
Reviews: 0 Publications and Reviews
(0 Publications and Reviews)

Click on the image to see the image legend

Single-Luciferase Reporter Assay Kit

Available Options

* Size:


Add to Shopping Cart Buy It Now GET A QUOTE

Documents

Datasheet SDS
Abbexa's Single-Luciferase Reporter Assay Kit is suitable for detection of firefly luciferase, a widely used as a reporter for studying gene regulation and function in mammalian cells and tissues due to its sensitivity and the absence of endogeneous luciferase activity in mammalian cells.

Contents:

Component 50 rxns 200 rxns
Luciferase Reaction Buffer5 ml 20 ml
Luciferase Reaction Substrate 1 vial 4 vials
5X Cell Lysis Buffer 5 ml 20 ml


Target Single-Luciferase Reporter Assay Kit
Form Liquid
Storage Store at -20°C in the dark. Avoid repeated freeze/thaw cycles.
Availability Shipped within 10-20 working days.
Note This product is for research use only. This product is shipped with dry ice.
Directions for use
  1. Prepare the 1X Cell Lysis Buffer by adding nuclease-free water to the 5X Cell Lysis Buffer at a ratio of 4:1 (e.g. add 20 ml of nuclease-free water to 5 ml of 5X Cell Lysis Buffer to prepare 25 ml of 1X Cell Lysis Buffer).
  2. Prepare the Working Luciferase Reaction Reagent by adding 5 ml of Luciferase Reaction Buffer to 1 vial of lyophilized Luciferase Reaction Substrate. Mix thoroughly and ensure that the lyophilized powder has completely dissolved. The Working Luciferase Reaction Reagent should be used within one day, or aliquoted and stored in the dark at -20°C (up to one month) or -70°C (up to one year).
  3. Wash cells twice with PBS and add 1X Cell Lysis Buffer. If using a well-plate, the table below shows the recommended volume of 1X Cell Lysis Buffer.
    Number of Wells Volume of 1X Cell Lysis Buffer/well
    6500 µl
    12 250 µl
    24 100 µl
    48 60 µl
    96 20 µl

    Incubate at room temperature for 10 minutes. Collect the cells by scraping. Centrifuge at 12,000 × g for 10 minutes at 2-8°C. Transfer the supernatant (containing the cell lysate) to a new tube.
  4. To 20 µl of cell lysate, add 100 µl of Working Luciferase Reaction Reagent. Measure the luminescence signal in a luminometer immediately.
Research Articles on Single-Luciferase Reporter Assay Kit


Write a review

Continue
Tags:
(Click to show)