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Target | Smith Antigen (Sm) |
Clonality | Polyclonal |
Reactivity | Human |
Tested Applications | ELISA |
Host | Human |
Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | Unconjugated |
Form | Lyophilized |
Purification | Purified from single donor human plasma. The plasma was delipidized and defibrinated, then an immunoglobulin fraction was precipitated and collected. |
Reconstitution | Reconstitute in 2 ml distilled water. |
Storage | Store at or below 0 °C. Avoid repeated freeze/thaw cycles. |
Buffer | Prior to lyophilization: 0.01 M Phosphate, 0.13 M Sodium Chloride, pH 7.2, containing 0.02% NaN3. |
Specificity | Shows the Sm precipitate band when tested using double immunodiffusion against rabbit thymus extract. Specificity is verified by identity of precipitation with CDC-AF reference serum. Anti-RNP precipitant is also detected. Titer will vary with test system; however, undiluted antibody shows a strong Sm precipitate band. Exhibits no reactivity at a dilution of 1/100 when tested against purified SSB, Scl-70 and Jo-1 antigens in an ELISA system designed to detect all IgG subclasses. Anti-RNP and low levels of anti-SSA activity are detected in this ELISA system. |
Concentration | Not determined. |
Availability | Shipped within 10-20 working days. |
Note | This product is for research use only. |
Directions for use | Centrifuge before opening to ensure complete recovery of vial contents. |