Mothers Against Decapentaplegic Homolog 2 (Smad2) Antibody

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Catalogue No: abx007233
Price: US$435.00
(Size: 100 µl)

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Western blot analysis of lysates from wild type (WT) and Smad2 knockout (KO) HeLa cells, using [KO Validated] Smad2 Antibody at 1/1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5min.
Mothers Against Decapentaplegic Homolog 2 (Smad2) Antibody

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Datasheet SDS
Smad2 Antibody is a Rabbit Polyclonal Antibody against Smad2.

Target Mothers Against Decapentaplegic Homolog 2 (Smad2)
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC, IF/ICC
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/500 - 1/2000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Smad2.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q15796 (UniProt, ExPASy)
UniProt Entry Name SMAD2_HUMAN
Gene Symbol SMAD2
GeneID 4087
NCBI Accession NP_005892.1
KEGG hsa:4087
String 9606.ENSP00000262160
Molecular Weight Calculated MW: 52 kDa
Observed MW: 60 kDa
Buffer PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Mothers Against Decapentaplegic Homolog 2 (Smad2)
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