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Mothers Against Decapentaplegic Homolog 2 Phospho-Thr220 (SMAD2 pT220) Antibody
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Catalogue No: abx032070
Price:
US$326.25
(Size: 80 µl)
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SMAD2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF?receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8) ; the common partner, (Smad4) that functions via its interaction to the various Smad's; and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.
Research Articles on Mothers Against Decapentaplegic Homolog 2 Phospho-Thr220 (SMAD2 pT220)
| Target | Mothers Against Decapentaplegic Homolog 2 Phospho-Thr220 (SMAD2 pT220) |
| Clonality | Polyclonal |
| Target Modification | Thr220 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, WB, IF/ICC, DB |
| Host | Rabbit |
| Recommended dilutions | WB: 1/500, IF/ICC: 1/10 - 1/50, DB: 1/500. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T220 of human SMAD2. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q15796 (UniProt, ExPASy) |
| UniProt Entry Name | SMAD2_HUMAN |
| NCBI Accession | NP_001003652.1 NP_005892.1 |
| KEGG | hsa:4087 |
| String | 9606.ENSP00000262160 |
| Molecular Weight | Calculated MW: 52.3 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Mouse, Rat, Cow and Zebrafish SMAD2. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |