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Syk (pT525 / 526) Antibody
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Catalogue No: abx031923
Price:
US$326.25
(Size: 50 µl)
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Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.
Research Articles on Syk (pT525/526)
| Target | Syk (pT525/526) |
| Clonality | Polyclonal |
| Target Modification | Thr525/526 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Host | Rabbit |
| Recommended dilutions | WB: 1/250, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y525/526 of human Syk. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P43405 (UniProt, ExPASy) |
| UniProt Entry Name | KSYK_HUMAN |
| NCBI Accession | NP_001128524.1 NP_001167638.1 NP_001167639.1 NP_003168.2 |
| KEGG | hsa:6850 |
| String | 9606.ENSP00000364907 |
| Molecular Weight | Calculated MW: 72.1 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Mouse, Rat and Pig SYK. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |