T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8A) Antibody

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Catalogue No: abx227102
Price: US$319.00
(Size: 40 µl)
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IHC-P analysis of CD8 expression in T-lymphocytes of the palatine tonsil (4 µm section).
T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8A) Antibody

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Datasheet SDS
T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8A) Antibody is a Rabbit Monoclonal antibody for the detection of CD8.

Target T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8A)
Clonality Monoclonal
Clone K487
Reactivity Human
Tested Applications IHC
Host Rabbit
Recommended dilutions IHC-P: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Synthetic peptide derived from the C-terminal region of human CD8.
Isotype IgG
Form Liquid
Purification Purified from rabbit antiserum by proprietary techniques.
Storage Store at 2-8°C.
UniProt Primary AC P01732 (UniProt, ExPASy)
Buffer 20 mM Tris-HCl, pH 8.0, containing 20 mg/ml BSA and 0.05% NaN3.
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Directions for use Suggested IHC-P Protocol
  1. Preparation of Tris-EDTA Buffer (10 mM Tris Base, 1 mM EDTA solution, 0.05% Tween-20, pH 9.0): Mix 1.21 g Tris and 0.37 g EDTA and dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1 M HCl and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
  2. Preparation of Wash Buffer: Use 0.05 M Tris-HCl, pH 7.6, containing 0.2% Tween-20.
  3. Deparaffinize the section in 3 changes of xylene, 5 minutes each.
  4. Wash the section in 96%, 80% and 70% ethanol, 10 minutes each.
  5. Rinse in distilled water.
  6. Block endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
  7. Rinse in distilled water.
  8. Antigen retrieval: immerse the slide in Tris-EDTA buffer, pH 9.0, and incubate in a water bath for 30-40 minutes at 96-98 °C.
  9. Remove the slide from the water bath and allow to stand at room temperature (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
  10. Rinse in distilled water.
  11. Wash in Wash Buffer for 5 minutes.
  12. Incubate the section with primary antibody at 1/100 - 1/200 dilution for 1 hour in a closed wet chamber. It is recommended to use abx291502 Primary Antibody Diluent or a diluent containing protease-free BSA (> 1 mg/ml) to dilute this antibody.
  13. Wash twice with Wash Buffer, 5 minutes each.
  14. Add the secondary antibody and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). It is recommended to use abx291501 Rabbit and Mouse HRP/DAB Detection Kit.
  15. Wash twice in Wash Buffer, 5 minutes each.
  16. Apply the DAB chromagen for 1-3 minutes.
  17. Wash in distilled water for 10 minutes.
  18. Stain in hematoxylin for 5 minutes.
  19. Wash in distilled water for 10 minutes.
  20. Dehydrate the section in 2 changes of 96% ethanol, 5 minutes each.
  21. Wash the section in 2 changes of xylene, 2 minutes each.
  22. Mount the slide for observation.
Research Articles on T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8A)


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