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Rat Tight Junction Protein ZO-1 (TJP1) Protein
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Catalogue No: abx165943
Price:
US$275.50
(Size: 10 µg)
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Rat Tight Junction Protein 1 is a recombinant Rat protein expressed in E. coli. This protein is the immunogen for the following antibodies: abx130397
Research Articles on Tight Junction Protein ZO-1 (TJP1)
| Target | Tight Junction Protein ZO-1 (TJP1) |
| Origin | Rat |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 90% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | F1M4A0 (UniProt, ExPASy) |
| UniProt Entry Name | F1M4A0_RAT |
| Gene Symbol | TJP1 |
| String | 10116.ENSRNOP00000014988 |
| Molecular Weight | Calculated MW: 56.4 kDa Observed MW (SDS-PAGE): 70 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Sequence Fragment | Gly23-Asp502 |
| Sequence | GIAISGGR DNPHFQSGET SIVISDVLKG GPAEGQLQEN DRVAMVNGVS MDNVEHAFAV QQLRKSGKNA KITIRRKKKV QIPVSHPDPD PVSDNEDDSY DEDVHDPRSG RGALANRRGE KSWARDRSAS RDRSLSPRSD RRSVASSQPA KPTKVTLVKS RKNEEYGLRL ASHIFVKEIS QDSLAARDGN IQEGDVVLKI NGTVTENMSL TDAKTLIERS KGKLKMVVQR DERATLLNVP DLSDSIHSAN ASERDDISEI QSLASDHSVR SHDRPPRRSQ SRSPDQRSEP SDHSTQSPQQ PSNGSLRSRE EERMSKPGAV STPVKHVDDH TPKAVEEVTV EKHEKQTPTL PEPKPVYAQV GQPDVDLPVS PSDGVLPNST HEDGILRPSM KLVKFRKGDS VGLRLAGGND VGIFVAGVLE DSPAAKEGLE EGDQILRVNN VDFTNIIREE AVLFLLDLPK GEEVTILAQK KKDVYRRIVE SD |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose. |
| Activity | Not tested |
| Concentration | Prior to lyophilization: 400 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |