TMB Substrate

REQUEST MORE INFO
Catalogue No: abx299722
Price: US$522.00
(Size: 10 × 100 ml)
Reviews: 0 Publications and Reviews
(0 Publications and Reviews)

Click on the image to see the image legend

TMB Substrate

Available Options

* Size:

Add to Shopping Cart Buy It Now GET A QUOTE

Documents

Datasheet SDS
TMB Substrate is a ready-to-use reagent for use in ELISA. It reacts with horseradish peroxidase (HRP) to produce a blue colour. The reaction can be terminated by adding acidic stop solution (sulphuric or hydrochloric acid), which produces a stable yellow reaction product with absorbance maxima at 450 nm.

Target TMB Substrate
Tested Applications ELISA
Storage Store in the dark between 2-8 °C.
Buffer Contains 3,3',5,5'-Tetramethylbenzidine (TMB).
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Directions for use
  1. Add 100 µl of TMB to each microplate well.
  2. Incubate in the dark at 37 °C for 20-30 min. The reaction can be terminated when the desired color develops.
  3. Add 100 µl of stop solution (0.3 M H2SO4 or 0.25 M HCl) to each well.
  4. Read the absorbance of each well at 450 nm within 15 minutes.

Notes:
  • 0.1% NaF can be used as the stop solution instead of 0.3 M H2SO4 or 0.25 M HCl; the absorbance of each well should be read at 650 nm if using 0.1% NaF.
  • Avoid contamination with oxidizing reagents.
  • This product should be used in the dark. Do not use in metal containers.
  • High HRP concentrations may cause the TMB to turn a green or red color and may cause precipitation to occur. If this occurs, it is recommended to reduce the HRP concentration in the sample or reduce the incubation time.
Research Articles on TMB Substrate


Write a review

Continue
Tags:
(Click to show)