Ubiquitin C-Terminal Hydrolase UCH37 (UCH37) Antibody

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Catalogue No: abx031537
Price: US$326.25
(Size: 80 µl)
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Ubiquitin C-Terminal Hydrolase UCH37 (UCH37) Antibody
Ubiquitin C-Terminal Hydrolase UCH37 (UCH37) Antibody Ubiquitin C-Terminal Hydrolase UCH37 (UCH37) Antibody

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Datasheet SDS
Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1.

Target Ubiquitin C-Terminal Hydrolase UCH37 (UCH37)
Clonality Polyclonal
Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC
Host Rabbit
Recommended dilutions WB: 1/1000, IHC-F: 1/50 - 1/100. Not tested in IHC-P. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen KLH-conjugated synthetic peptide between 56-87 amino acids from the N-terminal region of human UCH37 (UCHL5).
Isotype IgG
Form Liquid
Purification Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q9Y5K5 (UniProt, ExPASy)
UniProt Secondary AC Q5LJA6, Q5LJA7, Q8TBS4, Q96BJ9, Q9H1W5, Q9P0I3, Q9UQN2
UniProt Entry Name UCHL5_HUMAN
String 9606.ENSP00000356425
Molecular Weight Calculated MW: 37.6 kDa
Buffer PBS containing 0.09% sodium azide.
Specificity Predicted to react with Cow and Pig UCHL5.
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Ubiquitin C-Terminal Hydrolase UCH37 (UCH37)
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