Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3 (UCHL3) Antibody

Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1.

Target	Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3 (UCHL3)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 195-225 amino acids from the C-terminal region of human UCHL3.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P15374 (UniProt, ExPASy)
UniProt Secondary AC	B2R970, Q5TBK8, Q6IBE9
UniProt Entry Name	UCHL3_HUMAN
NCBI Accession	NP_001257881.1 NP_005993.1
KEGG	hsa:7347
String	9606.ENSP00000366819
Molecular Weight	Calculated MW: 26.2 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse, Rat, Cow and Pig UCHL3.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3 (UCHL3)

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