UDP Glucose Ceramide Glucosyltransferase (UGCG) Protein

Human, Mouse, Rat UDP Glucose Ceramide Glucosyltransferase (UGCG) Protein (Active) is an Active Recombinant Human, Mouse, Rat protein.

Target	UDP Glucose Ceramide Glucosyltransferase (UGCG)
Origin	Human, Mouse, Rat
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 95%
Reconstitution	Reconstitute in ddH₂O to a concentration up to 0.05 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Molecular Weight	Calculated MW: 16.1 kDa Observed MW (SDS-PAGE): 16 kDa
Sequence Fragment	Lys39-Leu171
Sequence	KK ATDKQPYSKL PGVSLLKPLK GVDPNLINNL ETFFELDYPK YEVLLCVQDH DDPAIDVCKK LLGKYPNVDA RLFIGGKKVG INPKINNLMP GYEVAKYDLI WICDSGIRVI PDTLTDMVNQ MTEKVGLVHG L
Tag	N-terminal His tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl and 5% Trehalose.
Activity	Active
Biological Activity	UDP Glucose Ceramide Glucosyltransferase (UGCG) is an enzyme which catalyzes the first glycosylation step in glycosphingolipid biosynthesis. It belongs to the glycosyltransferase 2 family. UGCG is widely expressed and transcription is upregulated during keratinocyte differentiation. Besides, Large Multifunctional Peptidase 2 (LMP2) has been identified as an interactor of UGCG, thus abinding ELISA assay was conducted to detect the interaction of recombinant human UGCG and recombinant human LMP2. Briefly, UGCG were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to LMP2-coated microtiter wells and incubated for 2 h at 37 ℃. Wells were washed with PBST and incubated for 1 h with anti-UGCG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 ℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of UGCG and LMP2 was shown in Figure 4, and this effect was in a dose dependent manner.
Endotoxin Level	< 1.0 EU/ml (LAL method)
Concentration	Prior to lyophilization: 500 µg/ml
Availability	Shipped within 5-7 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Research Articles on UDP Glucose Ceramide Glucosyltransferase (UGCG)

UDP Glucose Ceramide Glucosyltransferase (UGCG) Protein (Active)

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