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Target | Vasoactive Intestinal Peptide |
Origin | Human |
Expression | Recombinant |
Tested Applications | SDS-PAGE |
Host | HEK293 cells |
Form | Lyophilized |
Purity | > 95% (SDS-PAGE) |
Reconstitution | Reconstitute in sterile H2O to a concentration of 0.25 mg/ml. Do not vortex. |
Storage | Store at < -20°C. |
UniProt Primary AC | P01282-2 (UniProt, ExPASy) |
Molecular Weight | Calculated MW: 15.9 kDa Observed MW (SDS-PAGE): 17-35 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
Sequence Fragment | Ser21-Gly152 |
Tag | C-terminal His tag |
Buffer | 20 mM PB, 150 mM NaCl, pH7.4. |
Activity | Not tested |
Endotoxin Level | < 1.0 EU per µg as determined by the LAL method. |
Availability | Shipped within 5-15 working days. |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |