Vascular Cell Adhesion Molecule 1 (VCAM1) Antibody

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Catalogue No: abx000664
Price: US$217.50
(Size: 20 µl)
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Western blot analysis of lysates from HUVEC cells, using VCAM1 Antibody at 1/500 dilution. HUVEC cells were treated by Human Tumor Necrosis Factor-α (hTNF-α) (10 ng/ml) for 16 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
Vascular Cell Adhesion Molecule 1 (VCAM1) Antibody Vascular Cell Adhesion Molecule 1 (VCAM1) Antibody

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Datasheet SDS
Vascular Cell Adhesion Molecule 1 / CD106 (VCAM1) Antibody is a Rabbit Polyclonal antibody against Vascular Cell Adhesion Molecule 1 / CD106 (VCAM1). This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.

Target Vascular Cell Adhesion Molecule 1 (VCAM1)
Clonality Polyclonal
Reactivity Human, Mouse
Tested Applications ELISA, WB, IF/ICC
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/500 - 1/1000, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen A synthetic peptide corresponding to a sequence within amino acids 300-400 of human VCAM1.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P19320 (UniProt, ExPASy)
UniProt Secondary AC A8K6R7, B4DKS4, E9PDD1, Q6NUP8
UniProt Entry Name VCAM1_HUMAN
Gene Symbol VCAM1
GeneID 7412
NCBI Accession NP_001069.1
KEGG hsa:7412
String 9606.ENSP00000294728
Molecular Weight Calculated MW: 81 kDa
Observed MW: 95-120 kDa
Buffer PBS, pH 7.3, containing 0.09% sodium azide, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Vascular Cell Adhesion Molecule 1 (VCAM1)
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