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Tested Applications | WB |
Form | Liquid |
Storage | Store at 4 °C for up to one year, or at -20 °C for long-term storage. |
Availability | Shipped within 5-10 working days. |
Note | This product is for research use only. |
Directions for use | Protocol: 1. Wash the membrane with TBS-T 3 times, 10 min each time. Immerse the membrane into an appropriate volume of WB Stripping Buffer, incubate at room temperature for 15-30 min and shake slowly. (Note: For some antibodies, a washing time of 30-60 min may be required).2. Using tweezers, remove the membrane. Elute with TBS-T once and wash for 5 min. 3. Seal with skimmed milk powder or BSA. Notes: 1. For horseradish peroxidase systems, 5% skimmed milk powder should be used for each seal. For alkaline phosphatase systems, casein should be used for each seal.2. PVDF membrane is recommended with this product. A high amount of protein loss has been observed when using this WB stripping buffer with NC membrane. 3. This buffer can be used for WB detection with ECL similar chemiluminescence reagents. This buffer is not compatible with non-chemiluminescence reagents, such as DAB, NBT/BCIP. 4. Please wear personal protective equipment, such as lab coats, safety glasses and disposable gloves, when using this product. |