Methylosome Protein 50 (WDR77) Antibody

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Catalogue No: abx005387
Price: US$427.75
(Size: 100 µl)

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Western blot analysis of various lysates using WDR77 Antibody at 1/1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 1s.
Methylosome Protein 50 (WDR77) Antibody Methylosome Protein 50 (WDR77) Antibody Methylosome Protein 50 (WDR77) Antibody

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Datasheet SDS
WDR77 Antibody is a Rabbit Polyclonal antibody against WDR77. WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002.

Target Methylosome Protein 50 (WDR77)
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IF/ICC
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/500 - 1/1000, IF/ICC: 1/50 - 1/100. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 153-243 of human WDR77.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q9BQA1 (UniProt, ExPASy)
UniProt Secondary AC B3KMW6, B4DP38, Q3LID2, Q53FU2, Q6JZZ5, Q96GK4, Q9BWY3
UniProt Entry Name MEP50_HUMAN
Gene Symbol WDR77
GeneID 79084
NCBI Accession NP_077007.1
String 9606.ENSP00000235090
Molecular Weight Calculated MW: 37 kDa
Observed MW: 43 kDa
Buffer PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
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