Human X-Ray Repair Cross Complementing 5/6 Heterodimer (XRCC5-XRCC6) Protein

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Catalogue No: abx693934
Price: US$1,203.50
(Size: 50 µg)

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Datasheet SDS
Human XRCC5 & XRCC6 Heterodimer Protein is a recombinant Human protein expressed in Baculovirus-Insect Cells. A DNA sequence encoding the XRCC5 (P13010) (Met 1-Ile 732) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the XRCC6 (P12956) (Met 1-Asp 609) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

Target X-Ray Repair Cross Complementing 5/6 Heterodimer (XRCC5-XRCC6)
Origin Human
Expression Recombinant
Tested Applications SDS-PAGE
Host Insect
Form Lyophilized
Purity > 90% (SDS-PAGE)
Reconstitution Reconstitute in sterile distilled water to a concentration of 0.14 mg/ml. Do not vortex.
Storage Store lyophilized between -20 °C and -80 °C. After reconstitution, store between 4-8 °C for up to 1 week, or aliquot and store at or below -20 °C for up to 3 months. Avoid repeated freeze/thaw cycles.
Validity The validity for this protein is 12 months.
UniProt Primary AC P13010 (UniProt, ExPASy) P12956 (UniProt, ExPASy)
UniProt Secondary AC A8K3X5, Q0Z7V0, Q4VBQ5, Q53HH7, Q7M4N0, Q9UCQ0, Q9UCQ1, B1AHC8, Q6FG89, Q9UCQ2, Q9UCQ3
UniProt Entry Name XRCC5_HUMAN, XRCC6_HUMAN
Gene Symbol XRCC5, XRCC6
GeneID 7520 2547
OMIM 194364 152690
HGNC 12833 4055
KEGG hsa:7520 hsa:2547
Ensembl ENSG00000079246, ENSG00000196419
String 9606.ENSP00000375978 9606.ENSP00000352257
Molecular Weight Calculated MW: 72/85 kDa
Observed MW (SDS-PAGE): 70/85 kDa
Sequence Fragment XRCC5 (Met1-Ile732) and XRCC6 (Met1-Asp609)
Tag N-terminal His tag
Buffer Prior to lyophilization: 20 mM Tris, 500 mM NaCl, pH 8.0, containing 2.85% Trehalose, 10.07% Mannitol, 0.005% Tween-80 and 10% glycerol.
Activity Not tested
Endotoxin Level < 1.0 EU/µg protein (LAL method)
Availability Shipped within 5-15 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Centrifuge before opening to ensure complete recovery of vial contents.
Research Articles on X-Ray Repair Cross Complementing 5/6 Heterodimer (XRCC5-XRCC6)