XmaI Enzyme

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Catalogue No: abx071051
Price: US$754.00
(Size: 250 U)
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XmaI Enzyme

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Datasheet SDS
XmaI is expressed and purified from E. coli that carries the recombinant XmaI gene. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation. Fast digestion in 5-15 minutes with high fidelity.

Contents:

Component 250 U 500 U
XmaI 1 × 250 U 2 × 250 U
10X Buffer 250 µl 250 µl
10X DNA Loading Buffer 1 ml 1 ml


Target XmaI
Host E. coli
Quality Control
  • Ligation and re-cutting: After 10-fold overdigestion with XmaI, more than 95% of the DNA fragments can be ligated with T4 DNA ligase at 25 °C. Of these ligated fragments, more than 95% can be recut.
  • 16-Hour incubation: A 50 µl reaction containing 1 µg of DNA and 10 units of enzyme incubated for 16 hours results in the same pattern of DNA bands as a reaction incubated for 1 hour with 1 unit of enzyme.
  • Blue/White screening (Terminal integrity): A DNA vector is digested at a unique site within the lacZα gene with a 10-fold excess of enzyme, and then ligated, transformed and plated on X-gal/IPTG plate. Successful expression of the β-galactosidase indicates that lacZα gene remains intact after cloning. A blue colony represents an intact gene and a white colony represents an interrupted gene. To be Blue/White certified, enzymes must produce fewer than 3% white colonies.
  • Exonuclease activity: After incubation for 4 hours at 37 °C, a 50 µl reaction containing 100 units of enzyme and 1 µg 3H DNA releases less than 0.1% radioactive substance.
  • Endonuclease activity: After incubation for 4 hours at 37 °C, a 50 µl reaction containing 15 units of enzyme with 1 µg pBR322 RFI DNA results in less than 10% conversion from RFI to RFII.
Storage Store at -20°C. Stable for up to 2 years from date of receipt.
Molecular Weight 37.6 kDa
Buffer Storage Buffer: 20 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1.5 mM DTT, 0.4 mg/ml BSA, 50% glycerol.
10X Buffer: 100 mM Tris-Ac (pH 7.9), 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA.
Activity Active
Biological Activity One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37 °C in a total reaction volume of 50 µl.

Recognition site:
5’...C↓CCGGG...3’
3’...GGGCC↑C...5’
Availability Shipped within 10-20 working days.
Note This product is for research use only. This product is shipped with dry ice.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Directions for use Thaw the 10X Buffer and bring to room temperature before use. For ≤ 0.5 µg DNA, add 2 µl of 10X Buffer, 0.5 µl of XmaI, and nuclease-free water (variable volume) to the sample to make a final reaction system volume of 20 µl. For ≤ 1 µg DNA, add 5 µl of 10X Buffer, 1 µl of XmaI, and nuclease-free water (variable volume) to the sample to make a final reaction system volume of 50 µl. For > 2 µg DNA or incomplete digestion, increase the volume of enzyme. The total volume of enzyme should be less than 1/10 of the volume of the reaction system.

Incubate for 5-15 minutes at 37 °C. Inactivate the enzyme by adding 10X DNA Loading Buffer to a final concentration of 1X, or by heating at 65 °C for 20 minutes.

Note: Low ionic strength, high enzyme concentration, glycerol concentration > 5% or pH > 8.0 may result in star activity.
Research Articles on XmaI


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