Yellow Fever Virus Envelope Protein

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Catalogue No: abx620002
Price: US$1,160.00
(Size: 0.5 mg)

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Reviews: 1 Publications and Reviews
(1 Publications and Reviews)
SDS-PAGE analysis, with Coomassie blue staining, of virus generation tests. The negative control is marked Ø. Briefly, a pFastBac1 construction was used to transform E. coli strain DH10Bac to produce recombinant Bacmids. Purified recombinant Bacmids were then prepared and transfected in <i>Spodoptera frugiperda</i> (Sf) cells to generate P1 virus stock. The cells were collected by centrifugation. The supernatant (medium) was lysed in a buffer containing PBS, pH 7.5. After centrifugation, the soluble supernatnant fraction was collected (native protein extraction - NPE). The insoluble fraction was collected and solubilized with a denaturing buffer. After centrifugation, the supernatant was collected (denatured protein extraction - DPE). Two different clones, 1 and 2, were tested. The medium, NPE and DPE fractions were analyzed by SDS-PAGE.
Yellow Fever Virus Envelope Protein Yellow Fever Virus Envelope Protein Yellow Fever Virus Envelope Protein Yellow Fever Virus Envelope Protein Yellow Fever Virus Envelope Protein

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Datasheet SDS
Yellow Fever Virus Envelope (YFV E) Protein is a recombinant protein produced in Insect cells (Baculovirus).

Target Yellow Fever Virus Envelope Protein
Origin Virus
Expression Recombinant
Tested Applications SDS-PAGE
Host Insect
Form Lyophilized
Purification Purified by affinity chromatography using a nickel resin.
Reconstitution Reconstitute in ddH2O to a concentration of 1 mg/ml.
Storage Store at 4°C for short-term storage or at -20°C or -80°C for long-term storage. It is recommended to test freezing on small aliquots, and to store stock solutions at concentrations between 0.5-2 mg/ml. Glycerol (up to 50%) may be added for cryoprotection. Avoid repeated freeze/thaw cycles.
Stability Stability and storage tests were not performed.
UniProt Primary AC Q89293 (UniProt, ExPASy)
Sequence Fragment 1-391 AA
Sequence MESHMLLFLFSLATGLFGAVHG-AHCIGITDRD FIEGVHGGTW VSATLEQDKC VTVMAPDKPS LDISLETVAI DGPAEARKVC YSAVLTNVKI NDKCPSTGEA HLEEENEGDN ACKRTYSDRG WGNGCGLFGK GSIVACAKFT CAKSMSLFEV DQTKIQYVIR AQLHVGAKQE NWNTDIKTLK FDALSGSQEA EFTGYGRATL ECQVQTAVDF SNSYIAEMEK ESWIVDKQWA QDLTLPWQSG SRGVWREMHH LVEFEPPHAA TIKVLALGNQ EGSLKTALTG AMRVTKDTNN SKLYKLHGGH VACRVKLSAL TLKGTSYKMC TDKMSFVKNP TDTGHGTAVM QVKVPKGAPC RIPVMVADDL TAAVNKGILV TVNPIASTND DEVLIEVNPP FGDSYIIVGT GDSRLTYQWH KEGSSIGKLF TQTMKGAERL AVMGDAAWDF GSAGGFFTSV GKGIHTVFGS AFQGLFGGLS WITKVIMGVV LIWVGINTRN MTMSMSMILV GVIMMFLSLG VGA-GSHHHHHH
Tag C-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.5.
Endotoxin Level < 10 EU/ml
Concentration Prior to lyophilization: 0.50 mg/ml
Availability Shipped within 10-15 days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Yellow Fever Virus Envelope Protein
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