Yin Yang 1 (YY1) Antibody

Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.

Target	Yin Yang 1 (YY1)
Clonality	Monoclonal
Reactivity	Human, Rat, Monkey, Zebrafish
Tested Applications	ELISA, WB, IHC
Host	Mouse
Recommended dilutions	WB: 1/1000, IHC-P: 1/25. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Purified His-tagged Human YY1 protein
Isotype	IgG2a
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P25490 (UniProt, ExPASy)
UniProt Secondary AC	Q14935
UniProt Entry Name	TYY1_HUMAN
NCBI Accession	NP_003394.1
KEGG	hsa:7528
String	9606.ENSP00000262238
Molecular Weight	Calculated MW: 44.7 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Reacts with Human, Rat, Zebrafish and Cynomolgus Monkey YY1.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Yin Yang 1 (YY1)

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