Autophagy Related Protein 7 (ATG7) Antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

Target	Autophagy Related Protein 7 (ATG7)
Clonality	Polyclonal
Reactivity	Human, Mouse
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 284-313 amino acids from the Central region of human ATG7.
Isotype	IgG
Form	Liquid
Purification	Purified Rabbit Polyclonal Antibody.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	O95352 (UniProt, ExPASy)
UniProt Secondary AC	B4E170, E9PB95, Q7L8L0, Q9BWP2, Q9UFH4
UniProt Entry Name	ATG7_HUMAN
NCBI Accession	NP_001129503.2 NP_001138384.1 NP_006386.1
KEGG	hsa:10533
String	9606.ENSP00000346437
Molecular Weight	Calculated MW: 78 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Rat and Chicken ATG7.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Autophagy Related Protein 7 (ATG7)

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