Breast Cancer Type 2 Susceptibility Protein (BRCA2) Antibody

Breast Cancer Type 2 Susceptibility Protein (BRCA2) Antibody is a Rabbit Polyclonal antibody for the detection of BRCA2.

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SHFM1/DSS1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:24896180).

Target	Breast Cancer Type 2 Susceptibility Protein (BRCA2)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB
Host	Rabbit
Recommended dilutions	WB: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	breast cancer 2, early onset
Isotype	IgG
Form	Liquid
Purity	≥ 95% (SDS-PAGE)
Purification	Purified by immunogen affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Validity	12 months.
UniProt Primary AC	P51587 (UniProt, ExPASy)
UniProt Secondary AC	O00183, O15008, Q13879, Q5TBJ7
UniProt Entry Name	BRCA2_HUMAN
KEGG	hsa:675
String	9606.ENSP00000369497
Molecular Weight	Observed MW: 384 kDa
Buffer	PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.
Concentration	2 mg/ml
Availability	Shipped within 5-12 working days.
Note	This product is for research use only.

Research Articles on Breast Cancer Type 2 Susceptibility Protein (BRCA2)

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