Biotinidase (BTD) Antibody

BTD Antibody is a Rabbit Polyclonal antibody against BTD. The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described.

Target	Biotinidase (BTD)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000, IHC-P: 1/100 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 284-543 of human BTD.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P43251 (UniProt, ExPASy)
UniProt Secondary AC	A6NHF2, B2R865, B4DFX1, B4DLJ9, B7Z7C9, F8W1Q3, Q96EM9
UniProt Entry Name	BTD_HUMAN
Gene Symbol	BTD
GeneID	686
NCBI Accession	NP_000051.1
KEGG	hsa:686
String	9606.ENSP00000400995
Molecular Weight	Calculated MW: 61 kDa Observed MW: 61 kDa
Buffer	PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Biotinidase (BTD)

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