Dickkopf-Related Protein 2 (DKK2) Antibody

The 259-amino acid DKK2 protein, like DKK1, DKK3, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cys residues each. The second cys region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed expression of 4.0 and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15 to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.

Target	Dickkopf-Related Protein 2 (DKK2)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 225-251 amino acids from the C-terminal region of human DKK2.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q9UBU2 (UniProt, ExPASy)
UniProt Secondary AC	A0AVE9, B2R6S7, Q9UIU3
UniProt Entry Name	DKK2_HUMAN
String	9606.ENSP00000285311
Molecular Weight	Calculated MW: 28.4 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse DKK2.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Dickkopf-Related Protein 2 (DKK2)

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