EGFR (pY1125) Antibody

EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. A prototype member of the type 1 receptor tyrosine kinases, EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Target	EGFR (pY1125)
Clonality	Polyclonal
Target Modification	Tyr1125
Modification	Phosphorylation
Reactivity	Human
Tested Applications	ELISA, WB, DB
Host	Rabbit
Recommended dilutions	DB: 1/500. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1125 of human EGFR.
Isotype	IgG
Form	Liquid
Purification	Purified by protein A affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with peptides. The antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P00533 (UniProt, ExPASy)
UniProt Entry Name	EGFR_HUMAN
KEGG	hsa:1956
String	9606.ENSP00000275493
Molecular Weight	Calculated MW: 134 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on EGFR (pY1125)

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