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Human Sphingosine Kinase 1 (SPHK1) Protein
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Catalogue No: abx069144
Price:
US$290.00
(Size: 10 µg)
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Recombinant Sphingosine Kinase 1 (SPHK1) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).
Research Articles on Sphingosine Kinase 1 (SPHK1)
| Target | Sphingosine Kinase 1 (SPHK1) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 90% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q9NYA1 (UniProt, ExPASy) |
| UniProt Secondary AC | Q8N632, Q96GK1, Q9HD92, Q9NY70, Q9NYL3 |
| Molecular Weight | Calculated MW: 29.2 kDa Observed MW (SDS-PAGE): 32 kDa |
| Sequence Fragment | Leu148-Leu398 |
| Sequence | LLT NCTLLLCRRL LSPMNLLSLH TASGLRLFSV LSLAWGFIAD VDLESEKYRR LGEMRFTLGT FLRLAALRTY RGRLAYLPVG RVGSKTPASP VVVQQGPVDA HLVPLEEPVP SHWTVVPDED FVLVLALLHS HLGSEMFAAP MGRCAAGVMH LFYVRAGVSR AMLLRLFLAM EKGRHMEYEC PYLVYVPVVA FRLEPKDGKG VFAVDGELMV SEAVQGQVHP NYFWMVSGCV EPPPSWKPQQ MPPPEEPL |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
| Activity | Not tested |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |