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Introduction | Hyaluronic acid (HA, also known as hyaluronan) is a glycosaminoglycan – an unbranched polymer of disaccharides. In this instance, the HA glycosaminoglycan is a chain of alternating D-glucuronic acid and N-acetyl-D-glycosamine monomers, linked via β1,4 and β1,3 bonds. It is a major component of extracellular matrix and synovial fluid, and acts as a lubricant in the latter. HA interacts with the cell surface receptors CD44 and RHAMM, which promote cell adhesion and migration. High levels of hyaluronic acid are associated with some aggressive metastatic cancers. |
Target | Hyaluronic Acid |
Reactivity | General (All species) |
Tested Applications | ELISA |
Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
Validity | The validity for this kit is 6 months. |
Stability | The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout. |
Molecular Formula | (C14H21NO11)n |
Test Range | 6.25 ng/ml - 400 ng/ml |
Sensitivity | < 3.13 ng/ml |
Standard Form | Lyophilized |
Detection Method | Colorimetric |
Assay Type | Competitive |
Assay Data | Quantitative |
Sample Type | Serum, plasma and other biological fluids. |
CAS Number | 9004-61-9 |
Target Type | Antigen |
Assay Principle | This kit is based on competitive enzyme-linked immuno-sorbent assay technology. An antibody is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. A competitive inhibition reaction takes place between the biotin-labelled Hyaluronic Acid and the unlabelled- Hyaluronic Acid on the pre-coated antibody. The HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is added, only wells that contain sufficient Hyaluronic Acid will produce a blue coloured product, which then changes to yellow after adding the acidic stop solution. The intensity of the color yellow is inversely proportional to the Hyaluronic Acid amount bound on the plate. The OD is measured spectrophotometrically at 450 nm in a microplate reader, from which the concentration of Hyaluronic Acid can be calculated. |
Kit Components | The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
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Material Required But Not Provided |
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Reagent Preparation | This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
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Assay Procedure | This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
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Protocol | This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
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Results Calculation | This assay is competitive, therefore there is an inverse correlation between Hyaluronic Acid concentration in the sample and the absorbance measured. Create a graph with the log of the standard concentration (y-axis) and average absorbance measured (x-axis). Apply a best fit trendline through the standard points. The Hyaluronic Acid concentration of the samples can be interpolated from the standard curve. |
Availability | Shipped within 5-12 working days. |
Note | This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein. |
Standard | 400 ng/ml |
Intra Assay | CV ≤ 6.4% |
Inter Assay | CV ≤ 9.2% |