Mouse Articular Chondrocytes (AC)

Name: Mouse Articular Chondrocytes (AC)
Brand: Abbexa
SKU: abx700016
Price: 1240.0000 GBP
Availability: OnlineOnly

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Catalogue No: abx700016

US$1,798.00 US$899.00

(Size: 5 × 10^5 cells)

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* Size: 5 × 10^5 cells

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Datasheet SDS

Mouse Articular Chondrocytes (AC) are Adherent Mouse Chondrocytes from Mouse Articular cartilage.

Target	Articular Chondrocytes (AC)
Origin	Mouse
Host	Mouse
Storage	Shipped at -70 °C. Upon receipt, store in liquid nitrogen. Avoid repeated freeze/thaw cycles.
Validity	12 months.
Buffer	Contains 90% FBS and 10% DMSO.
Availability	Shipped within 10-20 working days.
Note	This product is for research use only. This product is shipped with dry ice. Not for human consumption, cosmetic, therapeutic or diagnostic use.
Directions for use	Recommended Cell Culture Conditions: DMEM + 10% FBS + Mouse Chondrocyte growth supplement + 1% Penicillin-Streptomycin Solution @ 37 °C, 95% air, 5% CO₂. Cell Recovery: Thaw cells in a 37 °C water bath with shaking until the mixture has dissolved. Transfer to a centrifuge tube and add culture medium (see Recommended Cell Culture Conditions above) at a volume 3-5 times the volume of the cells. Centrifuge at 1000 RPM for 5 minutes and discard the supernatant. Transfer to a T25 flask for culture. Suggested Cell Passage Procedure: Cells should be 85-95% confluent before cell passage is carried out. Discard the medium and wash with PBS 1-2 times. Add 1 ml of Trypsin at 37 °C, then observe the cells under a microscope. When the cells appear retracted and rounded, gently tap the culture flask to detatch the cells. Stop the trypsinization by adding 2 ml of culture medium containing 10% serum. Add fresh medium to resuspend the cells. The recommended ratio of primary cells is 1/2. Pipette to obtain a single cell suspension.

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