Mouse Collagen Type VII Alpha 1 (COL7A1) Protein

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Catalogue No: abx620130
Price: US$19,894.00
(Size: 20 mg)
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SDS-PAGE analysis of expression tests of the target protein. The negative control is marked Ø. Briefly, two E. coli strains (1 - BL21 (DE3), and 2 - T7E) were induced with 1 mM IPTG at either 16 °C for 16 h, or at 37 °C for 4 h. The bacterial cells were collected by centrifugation. The supernatant (medium) was lysed in a buffer containing PBS, pH 7.5, and 10% glycerol. After centrifugation, the soluble supernatnant fraction was collected (native protein extraction - NPE). The insoluble fraction was collected and solubilized with a denaturing buffer (8 M Urea). After centrifugation, the supernatant was collected (denatured protein extraction - DPE). The NPE and DPE fractions were analyzed by SDS-PAGE. Based on these tests, the optimal expression was observed in strain 1 - BL21 (DE3) cells incubated with 1 mM IPTG at 37 °C for 4 h, NPE fraction.
Mouse Collagen Type VII Alpha 1 (COL7A1) Protein Mouse Collagen Type VII Alpha 1 (COL7A1) Protein

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Datasheet SDS
Mouse Collagen Type VII (COL7) Protein is a Recombinant Mouse protein expressed in E. coli.

Target Collagen Type VII Alpha 1 (COL7A1)
Origin Mouse
Expression Recombinant
Tested Applications SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purification Purified by affinity chromatography using a nickel resin.
Reconstitution Reconstitute in ddH2O. Do not vortex.
Storage Store between 2-8 °C for short-term storage. Store between -20 and -80 °C for long-term storage. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q63870 (UniProt, ExPASy)
UniProt Entry Name CO7A1_MOUSE
Sequence Fragment 779-957 AA.
Sequence MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPLG S SV SKLQILNASS DVLRVTWVGV PGATSYKLAW GRSEGGPMKH RILPGNKESA EIRDLEGGVS YSVRVTALVG DREGAPVSIV ITTPPATPAL LETLQVVQSG EHSLRLRWEP VPGAPGFRLH WQPEGGQEQS LTLGPESNSY NLVGLEPATK YQVWLTVLGQ TGEGPPRKVT AYTEPSH EFGGGS HHHHHH
Tag N-terminal GST tag and C-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.5.
Activity Not tested
Endotoxin Level 10 EU/ml
Concentration Prior to lyophilization: 4 mg/ml
Availability Shipped within 2-3 months.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Research Articles on Collagen Type VII Alpha 1 (COL7A1)


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