Human Myelin Basic Protein (MBP) Protein

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Catalogue No: abx651548
Price: US$580.00
(Size: 50 µg)

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Datasheet SDS
Human Myelin Basic Protein (MBP) Protein is a Human protein expressed in a eukaryotic expression system (293F cells).

Target Myelin Basic Protein (MBP)
Origin Human
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host 293F cell
Conjugation Unconjugated
Form Lyophilized
Purity > 95%
Reconstitution To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P02686 (UniProt, ExPASy)
UniProt Entry Name MBP_HUMAN
KEGG hsa:4155
String 9606.ENSP00000380958
Molecular Weight Calculated MW: 34.7 kDa
Observed MW: 46 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Met1-Arg304
Sequence MGNHAGKREL NAEKASTNSE TNRGESEKKR NLGELSRTTS EDNEVFGEAD ANQNNGTSSQ DTAVTDSKRT ADPKNAWQDA HPADPGSRPH LIRLFSRDAP GREDNTFKDR PSESDELQTI QEDSAATSES LDVMASQKRP SQRHGSKYLA TASTMDHARH GFLPRHRDTG ILDSIGRFFG GDRGAPKRGS GKDSHHPART AHYGSLPQKS HGRTQDENPV VHFFKNIVTP RTPPPSQGKG RGLSLSRFSW GAEGQRPGFG YGGRASDYKS AHKGFKGVDA QGTLSKIFKL GGRDSRSGSP MARR
Tag N-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.4, containing 5% Trehalose.
Activity Not tested
Concentration Prior to lyophilization: 1500 µg/ml
Availability Shipped within 5-7 working days.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Research Articles on Myelin Basic Protein (MBP)


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