Human AMPK (G1/B1/A2) Heterotrimer Protein

Human AMPK (G1 Protein is a recombinant protein from Human produced in Baculovirus-Insect Cells. A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.

Target	AMPK (G1/B1/A2) Heterotrimer
Origin	Human
Expression	Recombinant
Tested Applications	SDS-PAGE
Host	Insect
Purity	> 94% (SDS-PAGE)
Storage	Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Validity	The validity for this protein is 6 months.
UniProt Primary AC	P54619 (UniProt, ExPASy)
UniProt Secondary AC	B4DDT7, Q8N7V9
UniProt Entry Name	AAKG1_HUMAN
KEGG	hsa:5571
String	9606.ENSP00000323867
Molecular Weight	158 kDa
Sequence Fragment	Met1-Pro331
Buffer	Supplied as sterile 50mM Tris, 200mM NaCl, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 10% gly, 1mM GSH, pH 7.4.
Activity	Active
Endotoxin Level	< 1.0 EU per µg of the protein as determined by the LAL method.
Availability	Shipped within 5-15 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on AMPK (G1/B1/A2) Heterotrimer

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